Exploring the impact of subarachnoid hemorrhage on neural tissue

Subarachnoid hemorrhage (SAH) is a type of stroke that occurs when there is bleeding in the subarachnoid space, the area between the brain and the skull. After the SAH, there is a secondary injury driving negative neurological outcomes, yet the molecular drivers of this neurological injury are unknown. Of particular concern, otherwise healthy, young women typically have worse outcomes after SAH, than men. However, sex disparities are unknown. To assess the impact of SAH on neural tissue health, we exposed cortical organoids to patient-derived cerebral spinal fluid (CSF) and observe that patient CSF impacts cortical organoids. Thus, using cortical organoids can help better understand the mechanisms driving SAH clinical outcomes and work toward effective treatments.